Basic Science

 

General Topic

Seminar Topic

Hrs.

Lecturer

The Central Dogma DNA to RNA to Protein 1.0 MOLEY

Conceptual Aspects

MOLEY
Basic Techniques in Recombinant DNA Technology Manipulation of Nucleic Acids

 

1.0 MOLEY
Common Expression Vectors

 

NELSON
DNA Cloning Genomic and cDNA Libraries 1.0 SCHEDL
Expression Cloning SCHEDL
Homologous Screening SCHEDL
Strategies / Examples / Pitfalls / Troubleshooting

 

SCHEDL
Common Techniques for Detection of Quantitation of Gene Expression Northern Blotting 1.0
Reverse Transcription — Polymerase Chain Reaction (RT/PCR)
Rnase Protection Assays

 

Concepts / Dos and Don’ts / Pros and Cons
DNA Protein Interactions Electrophoretic Mobility Shift Assays 1.0 NELSON
DNA Footprinting

 

NELSON
New Techniques Yeast One and Two Hybrid Screenings 1.0 SCHEDL
Differential Display and Subtraction Hybridization SCHEDL
Transgenic Models Knock-outs or Disruptions 1.0 MOLEY
Knock-ins MOLEY
Transgenic Over-expression MOLEY
Functional Genomics

 

Non-invasive Imaging 1.0 MOLEY
Non-invasive Molecular Probes MOLEY